Two key institutions helped shape the Villanella genre: the Santa Casa dell’Annunziata and the Accademia dei Sereni. The former was a lay institution renowned for its musical activity, where students were trained in chant, counterpoint, and improvisation. The latter played a political and intellectual role, influencing many musicians who were involved in its activities .

Moreover, Villanelle were a tool for cultural resistance. In the face of political and ecclesiastical repression, these songs gave voice to the Neapolitan people, asserting their cultural identity. The use of Neapolitan dialect in early Villanella collections was a deliberate choice to distance themselves from the dominant, imposed languages of the ruling class.